Efficient Enrichment of Hepatic Cancer Stem-Like Cells from a Primary Rat HCC Model via a Density Gradient Centrifugation-Centered Method

Efficient Enrichment of Hepatic Cancer Stem-Like Cells from a Primary Rat HCC Model via a Density Gradient Centrifugation-Centered Method

BACKGROUND Because few definitive markers are available for hepatic cancer stem cells (HCSCs), based on physical rather than immunochemical properties, we applied a novel method to enrich HCSCs. METHODOLOGY After hepatic tumor cells (HTCs) were first isolated from diethylinitrosamine-induced F344 rat HCC model using percoll discontinuous gradient centrifugation (PDGC) and purified via differe...

Sucrose density gradient centrifugation for efficient separation of engineered nanoparticles from a model organism, Caenorhabditis elegans

Motility and recovery of alpaca (Vicugna pacos) spermatozoa after centrifugation in a density gradient solution

Background: One of the factors limiting successful processing of alpaca (Vicugna pacos) semen is the viscosity of seminal plasma. The viscous nature of the collected ejaculate has hindered sperm cryopreservation as well as artificial insemination (AI) under field conditions. Aims: The objective of this investigation was to evaluate recovery, motility, ...

Enrichment and characterization of cancer stem-like cells from a cervical cancer cell line

Cancer stem cells (CSCs) are proposed to be responsible for tumor recurrence, metastasis and the high mortality rate of cancer patients. Isolation and identification of CSCs is crucial for basic and preclinical studies. However, as there are currently no universal markers for the isolation and identification of CSCs in any type of cancer, the method for isolating CSCs from primary cancer tissue...

Improvement of Spinal Cord Injury in Rat Model via Transplantation of Neural Stem Cells Derived From Bone Marrow

Abstract Background & Aims: Cell therapy is among the novel therapeutic methods effective in the treatment of spinal cord injuries. The aim of the present study was using neural stem cells (NSCs) in treating contusion spinal cord injury in rat model. Methods: Bone marrow stromal cells (BMSCs) were isolated from adult rats...